Lipids and Fatty acids

This platform includes yeast strains engineered for high level accumulation of fatty acids. These fatty acids and derived products can be used in different industries such as cosmetics, fragrances, flavours, lubricants, pharmaceuticals and food ingredients.

Using specialized enzymes and pathways, specific properties can be introduced into the products of interest, such as desaturation, chain length and functionalization.

The Blipid™ platform is compatible with our available YEASTsense™ biosensors for different precursors and products, allowing for rapid screening of high producers.

Relevant patent families

1. Shi S, Nielsen J. Methods and products for production of wax esters. Application no: PCT/SE2014/051229.
2. Siewers V, Buijs N, Zhou Y, Teixeira P, David F, Nielsen J. Engineering of hydrocarbon metabolism in yeast. Application no: PCT/SE2014/051229.
3. Siewers V, Krivoruchko A, Zhang Y, Dai, Z, Nielsen J. Engineering of Acetyl-CoA metabolism in yeast. PCT. Application no: PCT/SE2014/051228.
4. David F, Siewers V, Krivoruchko A, Wenning L, Yu T. Yeasts and Methods for Production of Very Long Chain Fatty Acid Derived Products. Application number: 62/142,236.

Relevant publications

1. Yu T, Zhou YJ, Wenning L, Liu Q, Krivoruchko A, Siewers V, Nielsen J, David F. “Metabolic engineering of Saccharomyces cerevisiae for production of very long chain fatty acid-derived chemicals.” Nat Commun. 8:15587 (2017)
2. Zhu Z, Zhou YJ, Krivoruchko A, Grininger M, Zhao ZK, Nielsen J. “Expanding the product portfolio of fungal type I fatty acid synthases” Nat Chem Biol. 13:360-362 (2017)
3. Wenning L, Yu T, David F, Nielsen J, Siewers V. “Establishing very long-chain fatty alcohol and wax ester biosynthesis in Saccharomyces cerevisiae” Biotechnol Bioeng. 114:1025-1035 (2017)
4. Zhou YJ, Buijs NA, Zhu Z, Gómez DO, Boonsombuti A, Siewers V, Nielsen J. “Harnessing Yeast Peroxisomes for Biosynthesis of Fatty-Acid-Derived Biofuels and Chemicals with Relieved Side-Pathway Competition” J Am Chem Soc. 138:15368-15377 (2016)
5. Zhou YJ, Buijs NA, Zhu Z, Qin J, Siewers V, Nielsen J. “Production of fatty acid-derived oleochemicals and biofuels by synthetic yeast cell factories” Nat Commun. 7:11709 (2016)
6. David F, Nielsen J, Siewers V. “Flux Control at the Malonyl-CoA Node through Hierarchical Dynamic Pathway Regulation in Saccharomyces cerevisiae” ACS Synth Biol. 5(3):224-33 (2016)
For a complete list of publications, see http://sysbio.se/Lab_Nielsen/publications.html
 

Ornithine and Polyamines

This platform includes yeast strains whose metabolism has been extensively engineered for high level production of the amino acid ornithine. Ornithine can be further converted into polyamines such as putrescine, spermidine and spermine.

These specialized polyamines not only function as chemical precursors and cosmetic ingredients but also have beneficial properties for promotion of longevity and treatment of age-associated conditions.

Relevant patent families

1. Qin J, Krivoruchko A, David F, Jiang B, Nielsen J. L-ornithine production in eukaryotic cells. PCT/SE2016/050193

Relevant publications

1. Qin J, Zhou YJ, Krivoruchko A, Huang M, Liu L, Khoomrung S, Siewers V, Jiang B, Nielsen J. “Modular pathway rewiring of Saccharomyces cerevisiae enables high-level production of L-ornithine” Nat Commun. 6:8224. (2015)
 

Recombinant Proteins

This platform includes yeast strains that have been engineered to enable production and secretion of high levels of heterologous proteins such as biopharmaceuticals and industrial enzymes.

Relevant publications

1. Wang G, Huang M, Nielsen J. “Exploring the potential of Saccharomyces cerevisiae for biopharmaceutical protein production.” Curr Opin Biotechnol. 48:77-84. (2017)
2. Huang M, Bai Y, Sjostrom SL, Hallström BM, Liu Z, Petranovic D, Uhlén M, Joensson HN, Andersson-Svahn H, Nielsen J. “Microfluidic screening and whole-genome sequencing identifies mutations associated with improved protein secretion by yeast” PNAS 112(34):E4689-96 (2015)
For a complete list of publications, see http://sysbio.se/Lab_Nielsen/publications.html