A recent publication in ACS Synthetic Biology by Florian David and Biopetrolia-affiliated researchers at Chalmers details the development of a fatty acid biosensor. The biosensor can ‘sense’ fatty acid levels in vivo, giving a fluorescent output. By using Fluorescence-Activated Cell Sorting (FACS), this biosensor can be used for screening millions of strains for beneficial modifications. In this publication, the biosensor was used to screen a library of genes for increasing fatty acid pools, identifying a number of novel engineering targets for increasing fatty acid levels.
Development of fatty acid-responsive biosensors for high-throughput screening of production strains