The use of 2A “self-cleaving” peptides can greatly simplify construction of complex pathways in yeast. However, not all 2A peptides are created equal. In an article published by researchers from Biopetrolia, Chalmers University and UNSEP, detailed characterization of multiple 2A peptide sequences to determine suitability for metabolic engineering applications in yeast was undertaken. The characterization pointed at particularly-efficient peptides, providing a new synthetic biology tool to allow rapid construction of metabolic pathways in yeast.
New synthetic biology tools by Biopetrolia and collaborators